[Thailand Darno Good News] Successful IVF for a Challenging Case: 14 Blastocysts Ignite New Hope

Male patient diagnosis: Congenital absence of the vas deferens—a structural anomaly preventing natural sperm egress. Intervention: Testicular sperm aspiration (TESA) to retrieve sperm directly from testicular tissue. Sperm processing: Two advanced cryopreservation techniques applied due to severe oligospermia—7 straws for low-sperm-volume freezing and 40 individually frozen spermatozoa for ICSI (intracytoplasmic sperm injection).

Male Patient Clinical Summary

Diagnosis:

Congenital absence of the vas deferens—an anatomical anomaly resulting in failure of sperm egress via natural pathways.

Intervention:

Testicular sperm aspiration (TESA) was performed to directly retrieve sperm from testicular tissue.

Sperm Processing:

Due to extremely low sperm count, two highly efficient sperm cryopreservation techniques were employed.

Low-volume sperm cryopreservation: 7 straws stored.

Single-sperm cryopreservation: 40 individual spermatozoa preserved for ICSI (intracytoplasmic sperm injection).

[图片:https://cdn.yun.sooce.cn/6/59417/png/1748416365653e4097925a6976eafb2f1aef40635c9e0.png?version=0][Sperm retrieval and cryopreservation workflow]

Female Patient Clinical Summary

Age: 38 years

Ovarian Function:

Baseline hormonal testing (FSH, LH, AMH) indicated adequate ovarian reserve, though age-related decline remains a concern.

Transvaginal ultrasound revealed a moderate antral follicle count (AFC), suggesting favorable responsiveness to ovarian stimulation.

Ovarian Stimulation Protocol:

A personalized Progesterone-Primed Ovarian Stimulation (PPOS) protocol was implemented to maximize oocyte quality while minimizing the risk of OHSS (ovarian hyperstimulation syndrome).

Oocyte Retrieval Outcome:

A total of 21 oocytes were retrieved, including 15 mature MII oocytes and 6 immature (GV/MI) oocytes.

All 15 mature oocytes underwent ICSI fertilization.

[图片:https://cdn.yun.sooce.cn/6/59417/png/1748416365652b0b5493cafe7d3a2778753d7885bcd57.png?version=1748416369][Oocyte retrieval and ICSI procedure]

Fertilization and Blastocyst Culture:

Of the 15 mature oocytes subjected to ICSI, 13 exhibited normal fertilization (2PN), while 2 displayed 1PN formation.

[图片:https://cdn.yun.sooce.cn/6/59417/png/17484163656553c3f29351555533dca146a6bc76ebc8e.png?version=1748416369][Fertilization assessment and embryo development monitoring]

The 6 immature oocytes underwent in vitro maturation (IVM); 5 successfully matured, all 5 fertilized (100% fertilization rate), and yielded 3 Day-5 blastocysts.

Ultimately, a total of 14 blastocysts were successfully cultured!

[图片:https://cdn.yun.sooce.cn/6/59417/png/1748416365687cea39a44e840c9b3f1f0cd7a0db9ec33.png?version=1748416369][Final blastocyst cohort: 14 high-quality blastocysts]

Key Technical Insights

How is sperm retrieved in cases of non-obstructive azoospermia?

Precise TESA combined with refined sperm cryopreservation techniques ensures optimal sperm viability and integrity.

How can advanced maternal age be overcome for successful conception?

A customized ovarian stimulation protocol paired with precise ICSI enables a 38-year-old woman to achieve high-quality blastocysts.

The Success Formula Behind 14 Blastocysts!

Integration of high-quality sperm, competent oocytes, and state-of-the-art embryo culture technology—underpinned by meticulous, end-to-end laboratory management—delivers exceptional success rates.

[图片:https://cdn.yun.sooce.cn/6/59417/jpg/1748416365699ea38a68d4e26bc3fab78c894ed17d50d.jpg?version=1748416369][World Fertility Industry Alliance (WFA) certification and laboratory excellence]

Darno Reproductive Medical Center, Thailand

Every embryo represents a family’s hope and dream

This article is a public-information summary published by WFA. It is intended for industry, media and the general public, and does not constitute medical or investment advice.