[Thailand Dhanura Success Story] From Struggles in Japan to Breakthrough at Dhanura Thailand

'In Japan, two cycles yielded 22 retrieved oocytes but only 3 mature ones! This time: 8 retrieved → 6 mature — maturity rate soared from 13.6% to 75%. So relieved we transferred here!' Comparative data between Japanese and Thai cycles.

'In Japan, two cycles yielded 22 retrieved oocytes but only 3 mature ones! This time: 8 retrieved → 6 mature — maturity rate soared from 13.6% to 75%. So relieved we transferred here!'

Comparative data between Japanese and Thai cycles

Basic Information

Female age: 41 years

Blood type: O positive (Rh+)

AMH: 1.46 ng/mL (range: 0.8–2.3)

Antral follicle count (AFC): ≥10

Menstrual cycle: Generally regular (26–28 days), occasionally prolonged

Past medical history / allergies / family genetic disorders: None

From 'Oocyte Producer' to 'Mature-Oocyte Hunter'

The Japanese Dilemma: The Overlooked 'Maturity Trap'

First Japanese cycle (July 2024): 8 follicles → 3 oocytes retrieved (including 1 MI immature oocyte) → 1 blastocyst (not PGT-A tested)

Second Japanese cycle (November 2024): 14 follicles → 3 mature oocytes (maturity rate: 21.4%) → 2 blastocysts (1 PGT-A tested and confirmed euploid)

Dhanura Thailand Breakthrough: A Shift from Quantity to Quality

Initial consultation at Dhanura Thailand (February 2025): Physician identified the key issue — 'Your AFC (≥10) is not poor, but oocyte maturation disorder is the primary factor.'

Dr. Xue’s strategy: 'There is room for improvement in ovarian stimulation — adopting the PPOS protocol to enhance oocyte maturity rate.'

Technical breakdown: How Dhanura achieved a 5-fold increase in maturity rate?

Three-Stage Maturity Management

Pre-treatment phase (prior to February 16, before arrival in Thailand): No pre-treatment required per physician assessment.

Stimulation phase (February 16–25)

Core elements of the PPOS protocol:

• Gradual stimulation: Starting dose of Gonal-F® 150 IU, increased to 225 IU later in the cycle

• Dydrogesterone to suppress premature LH surge (preventing premature ovulation)

• Addition of Cetrotide® 0.25 mg (GnRH antagonist) in mid-to-late stimulation phase

Oocyte retrieval phase (February 23–27)

Embryo culture and PGT-A screening

ICSI fertilization: All 6 mature oocytes successfully fertilized

Blastocyst formation: 3 blastocysts obtained (2 on Day 5, 1 on Day 6)

PGT-A chromosomal screening result: Euploid 5BB blastocyst (Day 5)

Key developmental milestones

MII oocytes: Only metaphase II (MII) oocytes possess fertilization competence

Cleavage synchrony: All Day-3 embryos were 8-cell stage, indicating consistent developmental potential

5BB blastocyst: '5' denotes degree of expansion; 'BB' indicates inner cell mass (ICM) and trophectoderm (TE) grading

Cross-border cycle: Shanghai–Bangkok 'Miracle Timeline' completed in just 9 days

Feb 16: Menstruation onset in Shanghai; PPOS protocol initiated

Feb 21: Transvaginal ultrasound at a top-tier Shanghai hospital: 7 follicles measuring 13–16 mm; E2 = 2150 pg/mL (indicating excellent follicular maturity)

Feb 23: Departure from Shanghai Pudong International Airport → Suvarnabhumi Airport, Bangkok; dedicated Dhanura Thailand staff met guest at airport and escorted to hotel; Dr. Xue monitored real-time updates on guest’s airport arrival and hotel check-in

Feb 24: Pre-retrieval follow-up consultation with Dr. Jariya at Dhanura Thailand

Feb 25: 'Oocyte protection' injection administered at Dhanura Thailand, based on follow-up findings

Feb 26: 'Trigger shot' (hCG or GnRH agonist) administered at Dhanura Thailand

Feb 27: Oocyte retrieval at Dhanura Thailand: 8 oocytes retrieved → 6 MII → ICSI performed on the same day

Expert Insight: Mature oocyte rate matters more than total oocyte count

Dr. Xue, Embryologist at Dhanura Thailand: 'For women over 40, the ovary resembles an 'aging orchard.' Rather than harvesting 10 green apples, it’s far better to wait for 3 ripe ones. Through precise hormonal modulation and rigorous maturity monitoring, we ensure each follicle reaches optimal maturity — 'fruit ripens and detaches naturally' at precisely the right moment.'

Medical Evaluation

For a 41-year-old woman with low AMH and limited ovarian reserve, this cycle represents a high-quality ovarian stimulation outcome. ICSI fertilization rate: 100%; blastocyst formation rate: 50%; one euploid blastocyst confirmed by PGT-A — clinically suitable for embryo transfer. Cycle management was highly efficient; cross-border patient journey was seamless; timing of trigger injection and ultrasound monitoring was well-coordinated.

This article is a public-information summary published by WFA. It is intended for industry, media and the general public, and does not constitute medical or investment advice.